Through this research, the optimal extraction conditions and characterization of flavonoids from the aqueous extract of the seeds of the Moringa olifera is studied. The effect of the extraction parameters such as PH, time of extraction, temperature and material ratio are studied to optimize the extraction conditions. Furthermore the total antioxidant capacity (TAC) of the optimum extract was studied using FRAP method and compared to the other extracts with different polarity. Further, the green synthesized silver nanoparticles by this method showed a very good stability and crystalinity which referes to the highly concentration of antioxidant phenolics through optimum extraction process.