Assessing hydroxyapatite biosolubilization by bacterial strains isolated from EL Halassa Khouribga P deposit.
Abstract
Natural phosphate represents an important reserve of phosphorus (Pi), essential element for plant nutrition and development. However, direct application of phosphate fertilizers has been proved ineffective for the phosphorus deficit soils remediation due to its low solubility. The aim of this study is to assess the ability of several bacteria species isolated from El Halassa, Khouribga deposit to solubilize inorganic phosphate. This ability has been tested and followed on NBRIP agar and broth medium with hydroxyapatite Ca5(PO4)3(OH) as sole phosphorus source. The results showed that Pseudomonas spp., P. cepacia and E. sakazakii presented the highest P solubilization efficiency (PSE) on NBRIP agar, with values of 79%, 77% and 60% respectively. Meanwhile, E. sakazakii, P. pseudomallei and P. cepacia showed the highest ability to release the soluble phosphate with concentrations of 1728 ± 0.5, 1432.2 ± 1 and 1272.1 ± 0.5 mg Pi L-1 respectively in the presence of Ca5(PO4)3(OH) as sole phosphorus source. Monitoring of the orthophosphates release in the medium for each strain, revealed that orthophosphates amounts progressively increase during first days of incubation and at the same time as the pH decreases. This can be explained by the secretion of organic acids and H+ protons by these microorganisms.