In this study, we will be studied quantitatively the binding chain role and understanding the interactions between diene (D) and dienophile (Dp) fragments in the intra-molecule (IM) and identifying the physicochemical variations of these fragments when passing from the isolated fragment (IF) to the linked fragment (LF) at the intra-molecule using the nucleophilic and electrophilic Fukui functions  calculated by DFT (density functional theory) method with the B3LYP hybrid and 6-31G(d,p) basis set level. The results found show that the binding chain has a low contribution to the physicochemical character of the intra-molecule and that his principal role focuses on the transfer of the charge between fragments, the quantitative studies we permit to conclude that the nucleophilic Fukui functions of Diene are remains unchanged in the linked fragment than that in diene (D) of isolated fragment (FD_IF) as (and the electrophilic Fukui function of dienophile is divided almost in half in the linked fragment than that in dienophile (Dp) in isolated fragment as .