The composition of honey is variable according to the current differences in plant types, climate, environmental conditions, and contribution of the beekeeper. Argania Spinosa is an endemic tree from southwestern Morocco, its famous argan oil is well known for its many healing properties including antioxidant and anti-inflammatory effects. This study aims to characterize for the first time the physicochemical, antioxidant, and antibacterial potential of Argania honey. Eight samples of honey were provided by beekeepers of the Tiznit Province. The pollen content was carried out to make the determination of Argania Honey and the characterization of the physicochemical parameters was carried out based on: HMF, pH, total polyphenol content, sugar composition, and diastase activity. The potentiality of Argania honey as an element antioxidant then bactericide agent. The outcomes data showed that the evaluated parameters are according to the guaranteed criteria quality of honey. Interestingly, the high polyphenols content (1295 mg GAE/Kg), presence of only reducing sugars (glucose and fructose), and the antibacterial action of Argania honey demonstrated by the average zone of inhibition between 7.7 mm and 12.07 mm for S. aureus, K. pneumonia and B. subtilis may be suitable its use for wound healing. The analysis of Argania honey revealed physicochemical, antioxidant, antibacterial, and wound healing properties that were approved according to standard criteria. These characteristics provide an important biological potential that may be reconsidered as a high-value commercial and therapeutic.