The research aims to evaluate the phytochemical constituents and biological activity (antibacterial and antioxidant activity) of the extract of endophytic fungi isolated from the seeds of Andrographis paniculata. Isolation of endophytic fungus followed the dilution method with Potato Dextrose Agar (PDA) media. Three single strains of fungi have been isolated from seeds of A. panicalata. They were cultivated on the white, black, and red rice media for three weeks, respectively. Extraction of the cultivated fungi with ethyl acetate followed by evaporation yielded the crude extract. The antibacterial and antioxidant activity were evaluated for nine crude extracts of fungi. Data of their biological activities showed that the ethyl acetate extract from fungus SAP-1 on the black rice media was the rich of active compounds. Molecular identification showing the fungus SAP-1 was Phyllosticta capitalensis. Further research of isolation and structure elucidation of bioactive compounds from fungal P. capitalensis obtained from the seeds of A. paniculata will be performed in the future. Interestingly, phytochemical and biological study of extract of endophytic fungus P. capitalensis from the seeds of A. paniculata is firstly reported in this study.