Vinasse waste is liquid waste from distillation products in bioethanol.Vinasse processes. The rest is organic matter which can be used to fertilize the soil. Therefore, alcohol waste must be treated with the addition of complementary organic materials in order to obtain it used as a plant media for probiotic bacteria which are very good for soil and plants.The production of probiotic bacteria culture media used the basic ingredients of vinasse waste mixed with rice bran and shrimp paste, which can work effectively to ferment organic matter. Bacteria will be cultured by adding vinasse waste and shrimp paste boiled for 5 minutes and cooled to room temperature 25^oC, let stand for 3 weeks (21 days). Cow manure and rabbit urine are potential ingredients for making organic fertilizers. Furthermore, making fertilizer from cow manure and rabbit urine using EM-4 bacteria cultured with vinasse using variationsin fermentation time of 7, 14, 21 days and sugar content of 0,25; 0,5; 0,625 (% w/v). Then the finished fertilizer sample is tested for analysis of the levels of Nitrogen, Phosphor, And Potassium, and C. Organic. Test nitrogen levels with the Kjeldahl method, analysis of phosphorus content (P₂O₅) and K (K₂O) levels with HNO₃ and HClO₄ extraction and C.Organic analysis using the Walkley & Black method. The results of the analysis test for the optimum levels of Nitrogen (N) obtained fermentation time of 21 days with a level of 1,14%. The percentage of phosphorus (P₂O₅) and and potassium (K₂O) obtained the best fermentation time of 14 days with levels of 0,51% and 0,74% respectively. The optimum conditions for Nitrogen (N) and potassium (K₂O) are in a 0,5% sugar concentration except for the optimum Phosphorus (P₂O₅) in a sugar concentration of 0,625%. The results of the analysis of fertilizers that meet the parameters of Indonesian National Standard 19-7030-2004 are the variation of fermentation time on the 21^st day with an N content of 1,14%; P 1,43%; K 0,42%; C. Organic 20,26; and the C / N ratio of 17,81.