The research has been conducted to determine the ability of C-4-methoxyphenyl-5,11,17,23-tetracarboxyl-calix[4]resorcinarene as antibacterial agent. The investigation has also studied the increasing its effectiveness due to the presence of Ag(1) in the calix complex. The antibacterial activity assay of the C-4-methoxyphenyl-5,11,17,23-tetracarboxyl-calix[4]resorcinarene and C-4-methoxyphenyl-5,11,17,23-tetracarboxyl-calix[4]resorcinarene-Ag(I) complex against Staphylococcus aureus and  Escherichia coli was carried out by measuring the inhibition zone diameter using the paper disc diffusion method. The result showed that the C-4-methoxyphenyl-5,11,17,23-tetracarboxyl-calix[4]resorcinarene its self does not have antibacterial activity toward Escherichia coli in various calix concentration of 10%, 15%, 20%, 25%, and 30%. However, the compound has moderate  antibacterial activity against Staphylococcus aureus. The existence of Ag(I) metal in calix compound complex cause the increasing in antibacterial activity for both of gram-positive bacteria, i.e. Escherichia coli, and gram-negative bacteria of Staphylococcus aureus. The complex calix-Ag(I) compound indicated strong response for both Staphylococcus aureus and  Escherichia coli.