This study was performed in the Gezira Area to investigate the organachlorine insecticide residues in raw cow’s milk. Five of raw cow’s milk samples have been collected from different places in Gezira Area, which suffered of previous health problems caused by unsafe use of pesticides. The samples were analyzed by applying different chromatographic methods so as to investigate Endsulfan and DDT residues. The findings showed that all investigated samples were found to contain endosulfan and DDT residues from 0.01 to 0.3 ppm for endosulfan and from 0.11 to 2.18 ppm for DDT respectively. It is important to mention that, the concentration of residues in some samples exceeded the standard levels were cited by FAO and WHO for acceptable daily intake as 0.006 ppm. for endosulfan and 0.002 ppm for DDT. This study recommended the need to prohibit the use of endosulfan in agricultural activities in Gezira Area, promote  health education programs among the farmers and encourage the routine pesticides residues studies in food.