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Because of the enormous range of biomedical uses and scientific interest in nanotechnology, the production of metallic nanoparticles using plant extracts is being examined. To investigate the antibacterial activity, silver nanoparticles (SNPs) were produced from aqueous silver nitrate by a simple and environmentally friendly approach employing leaf extract of Tarchonanthus Camphoratus (TC) as a reductant and stabilizer. A constant volume of plant extract (1mL) with various concentrations (1,2,4,6,8,10mM) of the aqueous silver nitrate (AgNO₃) was mixed separately to estimate their effects on the synthesized SNPs. The optical, surface morphological features, composition, and antibacterial activity of the SNPs were studied. The optical characteristics were evaluated at 429-446 nm using a UV spectrophotometer. Scanning Electron Microscopy (SEM) was used to examine the morphology. The analyzed SNPs were spherical in shape and well-distributed, with sizes ranging (21- 36 nm). Energy-dispersive X-ray spectroscopy (EDX) was used to estimate metal ion concentrations in the prepared SNPs. Using the cup-plate agar diffusion approach, the produced SNPs showed an inhibition zone that confirmed antibacterial effects against both Staphylococcus aureus and Escherichia coli bacteria. The maximum inhibition zones (13mm) for S. aureus and (13mm) for E.coli bacteria were observed. Finally, this study demonstrated that biosynthesized SNPs production using the described methodology was successful, as evidenced by several analysis methods, and demonstrated a new application of SNPs syntheses against the two types of bacteria growth.