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The aim of this study was to develop the analytical methods of iron through optimization reducing agent ability of iron (III) to iron (II) complex with formation of Fe (II) phenanthrolinee by spectrophotometry. Parameter of optimization that was used is   acetic buffer pH, time of the complex formation and reducing agent concentrations in the determination of iron level (content). Reducing agents are sodium tiosulfate (Na₂S₂O₃), hidroxylamine hydrochloride (NH₂OH.HCl) and sodium borohydride (NaBH₄). Results showed that the maximum wavelength of Fe (II)-Phenanthroline use all reducing agents is 510 nm, pH optimum of acetic buffer use reducing agent sodium tiosulfate (Na₂S₂O₃), hidroksilamin hydrochloride (NH₂OH.HCl) and sodium borohydride (NaBH₄) is 4.5. Optimum formation of indwelling time of iron (II) complex phenanthrolinee use reducing agent  Na₂ S₂ O₃ and NH₂OH.HCl is 15 minutes, whereas reducing agent NaBH₄ is 5 minutes.  Optimum concentration of reducing agent Na₂S₂O₃ that was used to reduce 5 ppm Fe(III) is 11 ppm, whereas for reducing agent NH₂OH.HCl is 8 ppm and reducing agent NaBH₄ is 150 ppm.