Comparative studies of antibacterial effect of 17 Members Macrocycle ligand and its Ni(II) complex with Herbal Essential Oils of Local Mentha Pulegium and Glycyrrhiza glabra on Staphylococcus aureus and Pseudomonas aeruginosa

Abstract

In the present study the essential oils of local Mentha pulegium and Glycyrrhiza glabra, as well as synthetic materials of 17 Members Macrocycle and its Ni(II) complex were selected to investigate their antimicrobial effect on Staphylococcus aureus ATCC 1341 and Pseudomonas aeruginosa ATCC 2785, as two common agents of nosocomial infections; to provide the solution with the lowest side effects to control or treat. The synthetic materials were synthesized and characterized by ¹H, ¹³C NMR, IR spectroscopy and elemental analysis. BHI broth and Blood agar mediums were used to recovery and purity of the bacteria. The chemical composition of the Hydro-distilled essential oils were analyzed by GC and GC/MS system and the antimicrobial activities were tested using well-diffusion assay and MIC and MBC values were estimated according to the Micro-dilution method. The analysis of M. pulegium oil showed the presence of 22 compounds, mostly Cineol (15.98%), Menthoforan (11.24%) and Pulegone (31.77%) and G. glabra oil contained 10 compounds, mainly (11.92%) γ-terpinene, (10.45%) Estragole, (14.89%) Isofenchon and (34.02%) Geranylhexanolate.