Phytochemical Screening and Biological Activity of Leaves and stems extract of Hammada Scoparia

Abstract

The antioxidant and antibacterial activities of alcoholic extract from wild Moroccan Hammada Scoparia leaves and stems were assessed. Phytochemical analysis revealed the presence of active ingredients such as Phenols, Flavonoids, Tannins, Alkaloids, Steroids and Saponins. Total content of phenol and flavonoid was quantitatively estimated in leaf and stem extracts of Hammada Scoparia. Total phenoilc content in the leaf and stem was found to be 50,08±1,09and 14,3±0,5 (mg EAG/g DM), while the total flavonoid content in the leaf and stem were found to be 28,8±0,07 and 5,1±0,2 (mg EQ/g DM) respectively. The antioxidant activity was determined using 1,1-diphenyl-2-picrylhydrazyl radical-scavenging DPPH assay, iron-reducing-power FRAP assay and inhibition test of the cation radical ABTS assay, the antioxidant activity showed that leaves extracts exhibited strong antioxidant activity. A linear relationship has been found between the total phenolic content and the antioxidant activity of the studied extracts. The extracts exhibited strong antibacterial activity against Citrobacter freundii and Acinetobacter baumanii. The stem extracts was more active than the leaf extracts against test bacteria with Inhibition zone diameters ranging from 7 to 20 mm and the leaf extracts from 7 to 13 mm. Hammada Scoparia leaves and stems had antiradical and antimicrobial activity that may be useful in pharmacy and phytotherapy.