Gene Editing Technology in the Treatment of Cancers and other Genetic Disorders

Authors

  • Mohammed TIMINOUNI Ecole des Hautes Etudes de Biotechnologie et de santé (EHEB)
  • Ahmed BENMOSTAPHA
  • Sofia FAIT
  • Lamiae ELKHATTABI
  • Mhammed CHAOUI ROQAI

DOI:

https://doi.org/10.80481/revuetest42353

Keywords:

Gene editing CRISPR/Cas9, Cancers, Viral particules, Genetic diseases

Abstract

The field of molecular biology has been transformed by the development of CRISPR/Cas9 gene editing technology, which has opened up previously unimaginable possibilities for the treatment of a variety of genetic diseases, including cancer. The development and potential uses of CRISPR/Cas9 gene editing technology to treat cancer and other genetic disorders are summarized in this abstract.

With the help of a The CRISPR/Cas9 system, which includes the Cas9 enzyme and guide RNA, precisely targets and modifies specific DNA sequences by directing Cas9 to a particular DNA sequence with the aid of a guide RNA molecule. This gene-editing tool is based on a natural bacterial defense mechanism and can perform modifications like gene knockout, gene insertion, or gene correction. The system allows for the selection of a specific DNA sequence based on location, function, or association with a particular gene or genetic trait. In particular, CRISPR/Cas9 technology holds great promise for understanding the underlying genetic mechanisms of tumorigenesis and devising innovative therapeutic strategies for cancer treatment. The detection and confirmation of oncogenes and tumor suppressor genes is one use of CRISPR/Cas9 in cancer research. Researchers can clarify the functional roles of particular genes in the initiation and growth of tumors by methodically focusing on these genes in cancer cells. Our understanding of cancer biology is improved by this information, which also offers potential targets for therapeutic intervention. Additionally, CRISPR/Cas9 gene editing has demonstrated significant promise for the creation of individualized cancer treatments. This technology can disrupt or correct cancer-related mutations through targeted gene knockout or correction, potentially restoring regular cellular functions. In the realm of genetic diseases, CRISPR/Cas9 gene editing offers a revolutionary approach to correct disease-causing mutations. Researchers hope to create effective treatments by precisely identifying and altering the genetic flaws that cause various inherited disorders. However, before CRISPR/Cas9 gene editing is widely used in medicine, there are still a number of obstacles to overcome. The main areas that need more research and improvement include off-target effects, delivery methods, and ethical considerations. However, the rapid development and ongoing improvements in CRISPR/Cas9 technology hold great promise for the creation of precise and targeted treatments for cancer and genetic disorders.

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Published

06-09-2023

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Section

Articles