Frequency of the toxic shock syndrome toxin-1 gene in methicillin-resistant Staphylococcus aureus isolated from Pediatric Hospital in Morocco


  • Mohammed SOBH
  • Abderrahmane HAMMOUNI
  • Mohammed TIMINOUNI
  • Mohamed EL AZHARI



resistant methicillin Staphylococcus aureus, Toxic shock syndrome toxin-1, Staphylococcus aureus.


Staphylococcus aureus is a common threat to hospitalized patients and is responsible for a variety of infections, ranging from superficial skin and soft tissue infections to toxic shock syndrome and severe systemic infections. Furthermore, methicillin- resistant Staphylococcus aureus (MRSA) has become a major concern in the hospital environment. Methicillin-resistant Staphylococcus aureus produces a range of virulence factors such as toxic shock syndrome toxin-1.

This study was conducted from December 2010 to Mai 2014 in a Mother Child Hospital CHU Mohamed VI Marrakech in Morocco. The Extreme Age was: 2 Days - 15 years, a total of 259 S. Aureus were collected from various clinical specimens, fifty three isolates identified as MRSA, In this study of 53 clinical SARM  isolates, the presence of the tst gene was assessed by polymerase chain reaction (PCR).

A total of  259 S. aureus were collected from various clinical specimens, fifty three isolates identified as MRSA, The study revealed 15/53 (28,30%) isolates were positive for the tst gene of methicillin-resistant S. aureus (MRSA) isolates.

These results reveal the remarkable risk of SARM infections in hospitals, regardless of methicillin- resistance status. Our results show a high rate of MRSA-TSST+ in the hospital.



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