Frequency of the toxic shock syndrome toxin-1 gene in methicillin-resistant Staphylococcus aureus isolated from Pediatric Hospital in Morocco

Authors

  • Mohammed SOBH
  • Abderrahmane HAMMOUNI
  • Mohammed TIMINOUNI
  • Mohamed EL AZHARI

DOI:

https://doi.org/10.80481/revuetest35786

Keywords:

resistant methicillin Staphylococcus aureus, Toxic shock syndrome toxin-1, Staphylococcus aureus.

Abstract

Staphylococcus aureus is a common threat to hospitalized patients and is responsible for a variety of infections, ranging from superficial skin and soft tissue infections to toxic shock syndrome and severe systemic infections. Furthermore, methicillin- resistant Staphylococcus aureus (MRSA) has become a major concern in the hospital environment. Methicillin-resistant Staphylococcus aureus produces a range of virulence factors such as toxic shock syndrome toxin-1.

This study was conducted from December 2010 to Mai 2014 in a Mother Child Hospital CHU Mohamed VI Marrakech in Morocco. The Extreme Age was: 2 Days - 15 years, a total of 259 S. Aureus were collected from various clinical specimens, fifty three isolates identified as MRSA, In this study of 53 clinical SARM  isolates, the presence of the tst gene was assessed by polymerase chain reaction (PCR).

A total of  259 S. aureus were collected from various clinical specimens, fifty three isolates identified as MRSA, The study revealed 15/53 (28,30%) isolates were positive for the tst gene of methicillin-resistant S. aureus (MRSA) isolates.

These results reveal the remarkable risk of SARM infections in hospitals, regardless of methicillin- resistance status. Our results show a high rate of MRSA-TSST+ in the hospital.

References

References

ALFATEMI, S. M. H., MOTAMEDIFAR, M., HADI, N. & SARAIE, H. S. E. J. J. J. O. M. 2014. Analysis of virulence genes among methicillin resistant Staphylococcus aureus (MRSA) strains. 7.

BARBOUR, S. D., SHLAES, D. M. & GUERTIN, S. R. J. P. 1984. Toxic-shock syndrome associated with nasal packing: analogy to tampon-associated illness. 73, 163-165.

DAGHISTANI, H. I., ISSA, A. A., SHEHABI, A. A. J. F. I. & MICROBIOLOGY, M. 2000. Frequency of nasal and wound isolates of Staphylococcus aureus associated with TSST-1 production in Jordanian population. 27, 95-98.

DEMIR, C., ASLANTAŞ, Ö., DURAN, N., OCAK, S. & ÖZER, B. J. T. J. O. M. S. 2011. Investigation of toxin genes in Staphylococcus aureus strains isolated in Mustafa Kemal University Hospital. 41, 343-352.

DINGES, M. M., ORWIN, P. M. & SCHLIEVERT, P. M. J. C. M. R. 2000. Exotoxins of Staphylococcus aureus. 13, 16-34.

FLORET, D., STAMM, D., COCHAT, P., DELMAS, P. & KOHLER, W. J. I. C. M. 1992. Streptococcal toxic shock syndrome in children. 18, 175-176.

JARRAUD, S., MOUGEL, C., THIOULOUSE, J., LINA, G., MEUGNIER, H., FOREY, F., NESME, X., ETIENNE, J., VANDENESCH, F. J. I. & IMMUNITY 2002. Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. 70, 631-641.

JIMÉNEZ, J. N., OCAMPO, A. M., VANEGAS, J. M., RODRÍGUEZ, E. A., GARCÉS, C. G., PATIÑO, L. A., OSPINA, S. & CORREA, M. M. J. M. D. I. O. C. 2011. Characterisation of virulence genes in methicillin susceptible and resistant Staphylococcus aureus isolates from a paediatric population in a university hospital of Medellin, Colombia. 106, 980-985.

LI, H., LLERA, A., MALCHIODI, E. L. & MARIUZZA, R. A. J. A. R. O. I. 1999. The structural basis of T cell activation by superantigens. 17, 435-466.

LLEWELYN, M. & COHEN, J. J. T. L. I. D. 2002. Superantigens: microbial agents that corrupt immunity. 2, 156-162.

MCCORMICK, J. K., YARWOOD, J. M. & SCHLIEVERT, P. M. J. A. R. O. M. 2001. Toxic shock syndrome and bacterial superantigens: an update. 55, 77.

MOMANI, O. A. A. 2021. INVESTIGATION OF STAPHYLOCOCCAL TOXIC SHOCK SYNDROM TOXIN-1 IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS.

MOTAMEDIFAR, M., EBRAHIM-SARAIE, H. S., ALFATEMI, S. M. H., ZALIPOUR, M., KAVEH, M. & KHOSHKHARAM-ROODMAJANI, H. J. R. D. S. B. D. M. T. 2015. Frequency of the toxic shock syndrome toxin-1 gene in methicillin-susceptible and-resistant Staphylococcus aureus isolates from teaching hospitals in Shiraz, Iran. 48, 90-93.

PECK, K. R., BAEK, J. Y., SONG, J.-H. & KO, K. S. J. J. O. K. M. S. 2009. Comparison of genotypes and enterotoxin genes between Staphylococcus aureus isolates from blood and nasal colonizers in a Korean hospital. 24, 585-591.

SABOUNI, F., MAHMOUDI, S., BAHADOR, A., POURAKBARI, B., SADEGHI, R. H., ASHTIANI, M. T. H., NIKMANESH, B., MAMISHI, S. J. O. P. H. & PERSPECTIVES, R. 2014. Virulence factors of Staphylococcus aureus isolates in an Iranian referral children's hospital. 5, 96-100.

SAMBROOK, J., FRITSCH, E. & MANIATIS, T. 1989. Extraction with phenol: chloroform. Molecular cloning: A laboratory manual, 3.

SCHLIEVERT, P. M., STRANDBERG, K. L., LIN, Y.-C., PETERSON, M. L., LEUNG, D. Y. J. J. O. A. & IMMUNOLOGY, C. 2010. Secreted virulence factor comparison between methicillin-resistant and methicillin-sensitive Staphylococcus aureus, and its relevance to atopic dermatitis. 125, 39-49.

SILA, J., SAUER, P. & KOLAR, M. J. B. P. M. F. U. P. O. C. R. 2009. Comparison of the prevalence of genes coding for enterotoxins, exfoliatins, panton-valentine leukocidin and tsst-1 between methicillin-resistant and methicillin-susceptible isolates of Staphylococcus aureus at the university hospital in olomouc. 153, 215-8.

TEYHOO, M., MOBAIYEN, H., AMIRMOZAFARI, N., MOADDAB, S. R. & MONESRAST, S. J. I. J. O. B. 2014. Prevalence of toxic shock syndrome toxin I producing clinical isolates of Staphylococcus aureus strains isolated from hospitals in Tabriz, Iran. 4, 169-176.

TRISTAN, A., YING, L., BES, M., ETIENNE, J., VANDENESCH, F. & LINA, G. J. J. O. C. M. 2003. Use of multiplex PCR to identify Staphylococcus aureus adhesins involved in human hematogenous infections. 41, 4465-4467.

TUFFS, S. W., HERFST, C. A., BAROJA, M. L., PODSKALNIY, V. A., DEJONG, E. N., COLEMAN, C. E. & MCCORMICK, J. K. J. M. M. 2019. Regulation of toxic shock syndrome toxin‐1 by the accessory gene regulator in Staphylococcus aureus is mediated by the repressor of toxins. 112, 1163-1177.

TURLEJ, A., HRYNIEWICZ, W. & EMPEL, J. J. P. J. O. M. 2011. Staphylococcal cassette chromosome mec (Sccmec) classification and typing methods: an overview. 60, 95.

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Published

15-12-2022

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