Seroprevalence of brucellosis among farmers in Morocco





farmers, brucellosis, seroprevalence, clinical symptoms, Rose Bengale, ELISA.


From17 to 31 December 2017, a cross‑sectional study was conducted by selecting a study population in numerous farms spred over 30 douars covering the entire province of Sidi Kacem. A total of 430 people were interviewed, using a structured questionnaire in Arabic languages including clinical symptoms.

A 3-5 ml venous blood sample was collected from each patient. Of all patients, 189 (44%) were male and 241 (56%) were femelle. Sample sera were tested for Brucella antibodies using rose Bengal plate test and immunoglobulin (Ig) M/IgG enzyme-linked immunosorbent assay (ELISA) for confirmation.

Of all people surveyed, 212 were confirmed positive for brucellosis, giving an overall seroprevalence of 49.30% (212/430). Of these, 128 (60.4%) were males and most of whom were married. However, the maritial status and educational level appears not to affect the seroprevalence of brucellosis for both sexes. Among patients who claim to have brucellosis related symptoms (n = 211), 101 subjects were IgG positive / IgM negative and 9 were IgM positive / IgG negative. However, 1 and 117 cases among those who profess not to have any symptoms of brucellosis were IgM positive /IgG negative and IgG positive / IgM negative, respectively.

It is concluded from the current study that brucellosis is highly prevalent in humans in Sidi Kacem province, where animal breeding is common. Preventive and control measures should immediately and strictly be implemented to protect animals and humans from brucellosis.


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