Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from Pediatric Hospital in Morocco

Authors

  • Bahija SERRAY
  • Sanaa BOURHIL ZRIOUIL Molecular Bacteriology Laboratory,Institut Pasteur du Maroc, Casablanca, Morocco.
  • Mohammed SOBH Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, Faculty of Sciences Ain-Chock, Route El Jadida, B. P. 5366, Casablanca, Morocco
  • Nabila SORAA Departments of Microbiology, University Hospital Mohamed VI, Marrakech, Morocco
  • Rachid SAILE
  • Rachid SAILE
  • Abderrahmane HAMMOUMI Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, Faculty of Sciences Ain-Chock, Route El Jadida, B. P. 5366, Casablanca, Morocco
  • Mohammed TIMINOUNI Molecular Bacteriology Laboratory,Institut Pasteur du Maroc, Casablanca, Morocco.
  • Mohamed El Azhari Medical Bacteriology Laboratory, Pasteur Institute of Morocco, Casablanca. Morocco

DOI:

https://doi.org/10.80481/revuetest34443

Keywords:

mecA gene, resistant methicillin Staphylococcus aureus, SCCmec Types.

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of infections acquired in both community and hospital settings associated with high morbidity and mortality worldwide. Resistance to methicillin in S. aureus is mediated by PBP2a, a penicillin-binding protein with low affinity to beta-lactams, encoded by the mecA gene. Accurate susceptibility testing of  S . aureus isolates and screening of patients for colonization with MRSA are important tools to limit the spread of this organism.

This study was conducted  from  December 2010  to Mai 2014 in a Mother Child Hospital CHU Mohamed VI  Marrakech in Morocco. The Extreme Age was: 2 Days - 15 years with an average age of  24 months, a total of  259 S . aureus were collected from various clinical specimens, fifty three isolates identified as MRSA using antibiogram, disc diffusion method, and PCR  to determine staphylococcal chromosome cassette mec (SCCmec).

All isolates from neonates were resistant to Penicillin, Oxacillin, all were susceptible to the Vancomycin, and Pristinamicin, 28 (52.83%) were resistant to Kanamycin, 27(50.94%) isolates were resistant to Pefloxacin, Tobramycin resistance was observed in 26 (49.06%) isolates, twenty four of isolates 53 (45.28%) were resistant to Erythromycin 21 (39.62%) to Gentamicin, Tetracycline resistance was observed in 19 (35.85%) isolates, resistance was also observed in 16 (30.19%) strains to both Rifampin and Trimethoprim-sulphamethoxazole, in 9 (16.98%) strains to fusidic acid, in 5 (9.43%) strains to Tigecycline, and in four strains (7.5%) to Chloramphenicol, only one (1.89%) strains to Lincomicin. SCCmec-III was the dominant SCCmec type (47.17%), followed by SCCmec-IV  (33.96%), SCCmec-II (11.32%), SCCmecV (5.66%) and SCCmec I (1.89%) were the less common,  SCCmec Type -VI  is absent in all strains.

The need for routine surveillance of child for resistant methicillin staphylococcus aureus to reduce child morbidity caused by the organism has therefore been revealed by this study.

Author Biographies

Bahija SERRAY

Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, Faculty of Sciences   Ain-Chock, Route El Jadida, B. P. 5366, Casablanca, Morocco

Molecular Bacteriology Laboratory,Institut Pasteur du Maroc, Casablanca, Morocco.


Sanaa BOURHIL ZRIOUIL, Molecular Bacteriology Laboratory,Institut Pasteur du Maroc, Casablanca, Morocco.

Molecular Bacteriology Laboratory,Institut Pasteur du Maroc, Casablanca, Morocco.

Mohammed SOBH, Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, Faculty of Sciences Ain-Chock, Route El Jadida, B. P. 5366, Casablanca, Morocco

Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, Faculty of Sciences   Ain-Chock, Route El Jadida, B. P. 5366, Casablanca, Morocco

Nabila SORAA, Departments of Microbiology, University Hospital Mohamed VI, Marrakech, Morocco

Departments of Microbiology, University Hospital Mohamed VI, Marrakech, Morocco

Abderrahmane HAMMOUMI, Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, Faculty of Sciences Ain-Chock, Route El Jadida, B. P. 5366, Casablanca, Morocco

Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, Faculty of Sciences   Ain-Chock, Route El Jadida, B. P. 5366, Casablanca, Morocco

Mohammed TIMINOUNI, Molecular Bacteriology Laboratory,Institut Pasteur du Maroc, Casablanca, Morocco.

Molecular Bacteriology Laboratory,Institut Pasteur du Maroc, Casablanca, Morocco.

Mohamed El Azhari, Medical Bacteriology Laboratory, Pasteur Institute of Morocco, Casablanca. Morocco

Medical Bacteriology Laboratory, Pasteur Institute of Morocco, Casablanca. Morocco

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08-11-2022

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