Role of molecular tools and serology tests in the diagnosis of the novel coronavirus: SARS-CoV-2

Authors

  • Salma MADIHI Molecular Biology Laboratory, Pasteur Institute of Morocco, 1, Place Louis Pasteur, Casablanca 20360, Morocco. Team of Experimental Oncology and Natural Substances, Cellular and Molecular Immunopharmacology, Sultan Moulay Slimane University, Faculty of Sciences and Technologies, Beni Mellal, Morocco
  • Warda BAHA Molecular Biology Laboratory, Pasteur Institute of Morocco, 1, Place Louis Pasteur, Casablanca 20360, Morocco
  • Fatiha LAZAR Molecular Biology Laboratory, Pasteur Institute of Morocco, 1, Place Louis Pasteur, Casablanca 20360, Morocco
  • Abdelmajid ZYAD Team of Experimental Oncology and Natural Substances, Cellular and Molecular Immunopharmacology, Sultan Moulay Slimane University, Faculty of Sciences and Technologies, Beni Mellal, Morocco
  • Abdelouaheb BENANI Molecular Biology Laboratory, Pasteur Institute of Morocco, 1, Place Louis Pasteur, Casablanca 20360, Morocco.

DOI:

https://doi.org/10.34874/PRSM.mjph-vol1iss1.24183

Keywords:

RT-PCR, SARS-CoV-2, COVID-19, Diagnosis, Serology tes

Abstract

Since the emergenceof severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV), it has become increasingly clear that bats are important reservoirs of coronaviruses. This investigation, with the apparition of the new coronavirus SARS-CoV-2 in Wuhan (China, December 2019), has shown 96.2% sequence similarity with the bat coronavirus. Through this review, we report the characterization of a SARS-CoV-2. The virus belongs to the Betacoronavirusgenera and shares less than 80% of identity with the SARS-CoV. This similarity was helpful to develop RT-PCR and other tools involved in SARS-CoV-2 RNA detection. We also report the importance of the different molecular tools and, the possible use of serology tests in the diagnosis of COVID-19. We discussed the strengths and weaknesses of each of these methods.

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Published

25-12-2020