SYNTHESIS OF PIPERAZINYL PEPTIDE CONJUGATES CONTAINING SMALLER PEPTIDE MOLECULES AS A PROMISING AVENUE FOR THE DEVELOPMENT OF NEW ANTIMICROBIAL AGENTS

Abstract

Novel piperazinypeptide conjugates were produced by combining shorter peptides with
benzylpiperazine using IBCF as the coupling agent, HOBt, and NMM as the base at -15 0C ±
1 0C. Initially, the process involved stirring the mixture at -15 0C ± 1 0C for 2 hours,
followed by an overnight reaction. The resulting compounds were identified using 1H NMR
and Rf values, and then tested for antibacterial effects against Staphylococcus aureus,
Escherichia coli, Klebsiella pnemoniae, and Pseudomonas aeruginosa, as well as antifungal
effects against Aspergillus Niger, Aspergillus flavus, and Fusarium monoliforme. XXI
displayed notable activity among the compounds synthesized, whereas XIX and XX showed
moderate activity.