In vitro anti-crystallizing activity of the fruit of the A. Unedo in the Human UrineIn vitro anti-crystallizing activity of the fruit of the A. Unedo in the Human Urine

Résumé

In Morocco, so-called forgotten fruits such as the fruits of the Arbutus Unedo can be valorized within the framework of regional sustainable development as a local and regional product. In addition, the prevalence of urolithiasis in the world is increasing rapidly. Urolithiasis involves the formation of crystalline aggregates called "urinary stones" that develop in the urinary tract, usually in the kidneys or ureters, but can also affect the bladder or urethra. The objective of this work is to evaluate, in vitro, the anti-crystallizing activity of the "forgotten fruit" of choice against calcium oxalate crystals in human urine.

Methods: the fruits of the Arbutus Unedo were collected from the Moulay Issa Ben Driss area of the Béni Mellal-Khenifra region. Samples of the fruit were crushed with a mortar so as to make a greater. Then, the samples were subjected to a cold aqueous maceration at 4°C for 48 hours. The study of the in vitro crystallization of calcium oxalate in human urine was carried out using the Polarized Light Optical Microscope (PLOM).

Results: the addition of the aqueous extract of the grater of the fruit of Arbutus unedo to human urine decreased the size and number of crystals of calcium oxalate di-hydrate and the fruit showed anti-lithiasis activity in vitro by inhibiting the growth of calcium oxalate crystals. Thus, inhibiting the aggregation of calcium oxalate crystals as a lithogenic species.