Isolation And molecular characterization of xylella fastidiosa from different host plant species in Italy (Apulia region)

Authors

  • Kaoutar El Handi Université Moulay Ismail / Faculty of Sciences Meknes
  • Marwa Mourou University of Tuscia
  • Khaoula Habbadi INRA/ Regional Center of Agricultural Research of Meknes
  • El Hassan Achbani INRA/ Regional Center of Agricultural Research of Meknes
  • Miloud Sabri Université Moulay Ismail / Faculty of Sciences Meknes
  • Majida Hafidi Université Moulay Ismail / Faculty of Sciences Meknes
  • Maroun El Moujabber CIHEAM/ Mediterranean Agronomic Institute of Bari
  • Franco Valentini CIHEAM/ Mediterranean Agronomic Institute of Bari

DOI:

https://doi.org/10.34874/IMIST.PRSM/afrimed-i137.36820

Keywords:

Xylella fastidiosa, OQDS, RT-LAMP, qPCR, Pure culture isolation, BCYE, Printing method, sap extraction method, MLST

Abstract

Pure culture isolation of the Xylella fastidiosa strain related to the olive quick decline
syndrome (OQDS), lately noticed in Apulia (southern Italy) was attempted from
naturally infected symptomatic plants mainly Olea europaea L., Polygala myrtifolia and Rosmarinus officinalis using printing and sap extraction methods. Prior to isolation, RTLAMP and qPCR were used to determine the presence of Xylella fastidiosa in all hosts. Pure bacterial cultures were obtained from both Olea europaea L. and Polygala myrtifolia extracts plated in buffered cysteine-yeast extract (BCYE) media. Two olive isolates were subsequently typed using the Multilocus Sequencing System (MLST). Findings indicate that beside Olea europaea, Polygala myrtifolia seems to have a good potential for isolation, and printing method has presented significantly better results. MLST shows genetic commonality with De Donno (ST53) strain and ascertain that Apulia region still have the same sequence type ST53 in the region.

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Published

30-12-2022

Issue

No. 137 (2022)

Section

Articles

License

As soon as the article is published, the author is deemed to have transferred his/her  rights to the publisher. Reproduction is under the terms of the Creative Commons Attribution - No Commercial Use - No Modification 4.0 International license : CC-BY-NC-ND