The Nelumbo nucifera naturally contains natural drugs and its suits to alternative for chemical drug diazepam. Results from the study revealed the fact of diazepam and lotus fresh petal extract possess a hypnotic/ sedative effect. In the present study, Nelumbo nucifera fresh petal extract in three different concentrations were tested against the Guppy fish, for the potential hypnotic effect for 10 days, and it concluded that fresh petal extract of lotus possesses a valuable hypnotic effect. The Guppy Fishes were treated with fresh petal extract of lotus and diazepam powder, after an hour of exposure they were assessed for locomotory behavior. However, guppy fish was exposed to fresh petal extract of lotus. In the present study, a maximum number was observed in the diazepam (19 out of 40) exposed fish indicating the strong sedative effect of diazepam powder. Moreover, lotus fresh petal extract 500mg/L was also reported to have a higher number of non-escaped fish (17 out of 40) next to diazepam states that lotus fresh petal extract also has similar sedative/ hypnotic nature as that of diazepam.