Comparative study of in-vitro antioxidant, anti-acetylcholinesterase, anti-xanthine oxidase, anti-5-lipoxygenase, anti-α-amylase and cytotoxic activity of Acacia species (Mimosaceae)
Abstract
The present study aimed the investigation of the acetylcholinesterase, xanthine oxidase (XOD), 5-lipoxygenase, α-amylase, antioxidant and cytotoxic activities of various organic extracts from seeds and pods of two Tunisian species of Acacia, A. cyclops and A. mollissima (Mimosaceae). At concentrations of 50 µg/mL, EtOAc extract of seeds from A. mollissima was able to strongly inhibit xanthine oxidase (XOD) with 98.40%. Moreover, EtOAc and n-BuOH extracts from pods of A. mollissima showed the highest inhibitor activity against 5-lipoxygenase with percentages of 79.60 and 64.86% inhibition, respectively. However, most of the tested extracts were not good acetylcholinesterase inhibitors. n-BuOH extracts of pods displayed the highest antioxidant activity (IC50= 36.03 to 45.90 µg/mL) for the DPPH assay and confirmed with the ABTS test. The eight extracts of Acacia species were tested for their cytotoxic activity in vitro using MCF-7, HCT-116, IGROV-1 and OVCAR-3 cell lines. The most active extracts were those of A. cyclops against IGROV-1 ranging from 12.9 to 16.1 µg/mL.