The essential oil of wild-growing medicinal specie Brocchia cinerea from oasis of Tata (Southeast of Morocco) was studied for chemical composition, antioxydant and antibacterial activities. The essential oil is extracted using a clevenger distillation apparatus. The antioxidant activity was determined by measuring the free radical scavenging power of DPPH and the antibacterial activity by broth dilution method Gas chromatography-mass spectrometry (GC/MS) results revealed that the major compounds of Brocchia cinerea are Thujone, Camphor and Eucalyptol. The antibacterial test of Brochia cinerea essential oil revealed a strong antibacterial activity against Listeria innocua, Pseudomonas aeruginosa and Staphylococcus aureus (MIC<1 µL) and also a high scavenging effect of DPPH (IC₅₀=0.080± 0.014).