Senna alexandrina pods and leaves have been used in herbal medicine since ancient times. The extracts of this plant contain mainly anthraquinone glycosides that have a significant laxative effect. In this study, the sennosides A and B, the major constituents of Senna alexandrina were calculated via a simple and accurate HPLC method. The yield of sennoside A was found 1.22 and 1.83% in the pods and leaves, respectively. Whereas the yield of sennoside B was found 2.7 and 1.83% in the pods and leaves, respectively.