Investigation of antibacterial and hepatoprotective activity of Lectin protein from Lepidium sativum seeds


  • Hamid Bashir 1Center for Applied Molecular Biology, 87-West canal, Bank Road, University of the Punjab



Lectins are oligomeric proteins that bind carbohydrates and take part in several biological recognition processes. Lectins are capable to accomplish various physiological functions like; anti-fungal, anti-viral, anti-tumor and cell agglutination. A lectin protein having 30kDa size was isolated from seeds of Lepidium sativum using ion chromatography on Q-Sepharose. Its antibacterial and anti-cancer potential against HepG2 cells was examined. Lectin protein exhibited a positive inhibitory effect against Escherichia coli, Staphylococcus aureus and Streptococcus pyrogenes with maximum inhibition zones of 15 & 23mm, 10 & 17mm and 12 & 18mm zone sizes respectively. The cytotoxicity of lectin purified from seeds of Lepidium sativum was evaluated on the HepG2 cells. The purified lectin protein inhibited the hepatocellular carcinoma cells (HepG2) growth with an IC50 of 265μg/ml. The finding of the current study highlights a promising anti-cancerous and anti-bacterial action of lectin purified from Lepidium sativum plant seeds






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