GC/MS Analysis of Phytoconstituents and Antimicrobial Evaluation Leaf Extract of Garcinia imberti Bourd


  • R. Ramasubbu Department of Biology, The Gandhigram Rural Institute (Deemed to be University), Gandhigram, Dindigul, Tamil Nadu




Garcinia imberti Bourd. is a strict endemic and endangered tree species belonging to the family Clusiaceae (Guttiferae), locally known as Manjakanji. The trees were over exploited for its unknown multi-usage valuable chemical compounds. The present study has been attempted to investigate the GC/MS analysis of chemical composition and antimicrobial activity of leaf extracts of Garcinia imberti. Powdered plant leaves were subjected to successive extract with petroleum ether, chloroform and methanol by soxhlet extraction method. Totally 24 different compounds from methanolic leaf extract were identified, Squalene, Supraene, Phenol, Dinoctyl phthalate, Gitoxigenin, Digitoxin and Bis (2ethylhexyl) Phthalate are found as dominate. In addition, antimicrobial activity was also studied using agar well diffusion method at various concentrations (100 µg/ml, 150 µg/ml and 200 µg/ml) of petroleum ether, chloroform and methanolic extracts. These extract inhibited severely on the growth of microorganisms, and implied antimicrobial activity on gram-positive bacteria, gram-negative bacteria and fungi. These extract was effectively tested against microorganisms which was well comparable with standard antibiotics (Ampicillin for bacteria and Amphotericin B for fungi). Among these three extracts evaluated methanol extract showed higher inhibitory activity against all the tested pathogenic bacteria and fungi and these three concentrations of the extracts tested at higher concentration (200 µg/ml). The high degree of antimicrobial activity seems to confirm the folk therapy of infections and traditional therapeutic claims of this rare tree.






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