Antimicrobial activity of the leaves essential oil of Eucalyptus camaldulensis Dehnh

M.H. Arwa, Z.A Aisha, A. Haidar


Essential oil from the leaves of Eucalyptus camaldulensis Dehnh. belonging to the family Myrtaceae was tested for its antimicrobial activity against six standard bacteria, two Gram positive bacteria (Bacillus subtilis, Staphylococcus aureus), four Gram negative (Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa) and two standard fungi (Aspergillus niger, Candida albicans) using the cup plate agar diffusion method. The essential oil of E. camaldulensis showed high activity (22mm) against S.aureus, (20mm) against E.coli and C. albicans. It also possessed moderate  activity (17mm)   against  B.subtilis, Kl. Pneumoniae and  Pr. vulgaris ,(18mm)  against Ps. aeruginosa and (16mm)  against A.niger. The minimum inhibitory concentrations (MICs) of E. camaldulensis essential oil against standard bacteria were determined using the agar plate dilution method and the MICs were12.5 µg/ml. The essential oil of E. camaldulensis was further tested against fifty clinical isolates, collected randomly from patients of Khartoum Teaching Hospital laboratory and Soba Hospital. The antibacterial activity of two reference drugs and the antifungal activity of two reference drugs were determined against six bacteria and two fungi and their activities were compared with the activity of essential oil of E. camaldulensis leaves. The essential oil of E. camaldulensis leaves can be of potential use as antimicrobial agents.


Antimicrobial activity, Essential oil, Eucalyptus camaldulensis, Leaves, Minimum Inhibitory Concentrations.

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ISSN: 2458-5920