Antimicrobial activity of fruits, leaves, seeds and stems extracts of Ziziphus spina -Christi

Aisha B. Ali, Aisha Z. Almagboul, Omer M. Mohammed


Petroleum ether , chloroform , methanol and aqueous extracts of the fruits, leaves, seeds and stems of  Ziziphus spina -Christi belonging to the family Rhamnaceae were screened for their antimicrobial activity against six standard bacteria: two Gram positive (Bacillus subtilis ,Staphylococcus aureus),four Gram negative (Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas aeruginosa) and two fungi (Aspergillus niger and Candida albicans)  using the cup plate agar diffusion method. The methanol extracts of all parts showed the highest activity against the bacteria tested followed by chloroform then petroleum ether. The aqueous extracts of all parts were inactive against all bacterial organisms. All four parts of the plant extracts showed no antifungal activity against the two fungi tested. The minimum inhibitory concentrations of the most active methanol extracts of fruits, leaves and stems were determine against standard bacteria using the agar plate dilution method. The antibacterial activity of five reference drugs and the antifungal activity of two reference drugs were determined against six bacteria and two fungi and their activities were compared with the activity of the plant extracts.


methanol extract, pathogens, Ziziphus spina – Christi L.

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ISSN: 2458-5920