Isolation of rutin anti inflammatory agent from ethanolic leaf extract of Rivea hypocrateriformis (Desr.) choisy

Authors

  • shanthini nachiar srm university
  • Dr P.R.KUMAR srm college of pharmacy

DOI:

https://doi.org/10.48347/IMIST.PRSM/ajmap-v9i2.38619

Keywords:

, HPTLC studies, Marker compound, column chromatography, Rivea hypocrateriformis ethanolic leaf extract, TLC plates

Abstract

Introduction: To extract the leafy parts with ethanol and perform the HPTLC studies with marker compounds Quercetin, Rutin, and gallic acid , a compound was isolated and susceptible to spectroscopic analysis. Background: Rivea hypocrateriformis (Desr.) Choisy holds Convolvulaceae family the leafy parts underwent a Soxhlet extraction with ethanol which was then subjected to a phytochemical investigation that shows the presence of alkaloids, phenols, flavonoids, terpenoids, tannins, glycosides saponins, sterols, and carbohydrates. Material and methods: Firstly, the ethanolic leaf extract is treated with petroleum ether to remove excess lipids, sterols, and impurities. TLC was performed by toluene: ethyl acetate: formic acid and the same solvent system was used for column chromatography. The bands getting separated into the column were collected separately in a test tube. Once the solvent toluene ends, the next solvent system with 18 ml of toluene and 2 ml of ethyl acetate, is introduced into the column and continues the separation procedure. Collect separated bands and perform TLC of each separate band by using the same solvent system in a concentration of 5:4:1. A single component on a TLC plate with a solvent system of 12 ml toluene and 8 ml ethyl acetate was obtained whose characterization was done by IR, NMR, and mass. Results:The RF value of Rutin is 0.17 which correlates with the Retention factor of ethanolic leaf extracts of RH by HPTLC comparative studies and a compound was isolated by column chromatography. Conclusion: These studies have concluded that a compound is separated by means of HPTLC and spectroscopic interpretation and identified as Rutin.

Author Biographies

shanthini nachiar, srm university

pharmacy

Dr P.R.KUMAR, srm college of pharmacy

pharmacy

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Published

17-10-2023

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Section

Research Articles