Antioxidant properties and hepatoprotective effect of Croccus sativus stamens against carbon tetrachloride‑induced liver injury in rats

Samira MAMRI, NourElhouda DAOUDI, Mohamed MARGHICH, Sabir OUAHHOUD, Amine KHOULATI, Mohammed CHOUKRI, Abdeslam ASEHRAOU, Mohamed BNOUHAM, Ennouamane SAALAOUI


Background: Carbon tetrachloride (CCl4) is one of the commonly used models for the induction of liver injury and screening of the hepatoprotective activity of different drugs. The aim of this study was to investigate the hepatoprotective activity and possible underlying mechanisms of methanolic and hydroethanolic extracts of Croccus sativus stamens using a model of carbon tetrachloride-induced liver injury in rats. Methods: Methanolic and hydroethanolic extracts was daily orally administered to rats at the doses of 250 mg/kg, prior to double intraperitoneal injection of 1% CCl4 on 7 days and 14 days. Their activities were compared with silymarin (250 mg/kg) for 14 days. Parameters of hepatic and renal function, lipid profile, and oxidative stress were evaluated in rat serum and tissues. Results: The results indicated that CCl4 injection in rats increased plasma aspartate aminotransferase (AST), Alanin aminotransferase (ALT), and Alkaline Phosphatase (ALP) activities, which were normalized by methanolic, and hydroethanolic pretreatment. In addition, administration of both extracts with CCl4 significantly inhibited alterations of kidney indices in the serum levels of creatinine and urea. Moreover, CCl4 administration increased the lipid peroxidation level (MDA) in tissues that is signifanctly reduced by the Methanolic and hydroethanolic extract of the plant. Methanolic and hydroethanolic treatment improved hepatic antioxidant status, by the elevation of the CAT and GSH level in CCl4‑administered rats. Conclusion: These findings demonstrated the potential effect of Croccus sativus stamens as a protector against liver injury. Mediated possibly by their antioxidant properties. Which are comparable to those of the standard hepatoprotective compound silymarin.


Carbon tetrachloride; Croccus sativus, stamens; hepatoxicity; hepatoprotective activity.

Full Text:



Copyright (c) 2022 Arabian Journal of Medicinal and Aromatic Plants

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

ISSN: 2458-5920