Extraction, Purification and HPLC quantification of Ursolic acid from leaves of Nepal cultivar of Rosemary officinalis.

Hemanth kumar Manikyam


Rosmarinus officinalis is a woody perennial Mediterranean shrubby herb with fragrant needle like green leaves. It’s a member of mint Lamiaceae family which includes many other herbs which grows up to 1.5 m tall on altitudes above 750 feet high. From ancient times it been used to treat many illnesses and culinary purpose, thus it is cultivated worldwide. Rosemary extracts are high in demand due to high natural antioxidant property obtained from Carnosic, Carnosol and other Triterpenoids like Ursolic, betulinic and Oleanolic acids. Ursolic acid is widely used in cosmetic industry because of its broad biological activities like anticancer, anti-inflammatory, anti-microbial and hepatoprotective activities.  Thus, we have carried out traditional solvent extraction and purification of Ursolic acid using ethanol and acetone as solvents. In this research work an easy and effective HPLC UV method quantification of Ursolic acid was carried out at 210 nm, flow rate of 1ml/min, injection volume of 10 µL, through C18 reverse phase column. Ursolic acid purity obtained was 50% with a retention time of 21.39 minutes. Thus, purity of 50% Ursolic acid obtained by solvent extraction method with recovery yield of above 98%. This is first time we are reporting method of analysis and extraction of Ursolic acid content from Nepal Cultivar of Rosemary officinalis.


Rosemary officinalis, Leaves, solvent extraction, Ursolic acid, Triterpenoids, Carnosic acid. HPLC quantification, Betulinic acid, Oleanolic acid

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DOI: https://doi.org/10.48347/IMIST.PRSM/ajmap-v9i2.29213

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ISSN: 2458-5920