The evaluation of antibacterial and antiproliferative activities of Rosmarinus officinalis essential oil from southern Morocco

Mounia Cherki, Mounchid Karima, Dersi Noureddine, Habti Noreddine, Alaoui Ismaili Mustapha, Aboussaouira Touria


Essential oils of plants contain many bioactive molecules and have recently a considerable place of application in pharmaceutical, cosmetic and food industry. The aim of this work is to study antibacterial and antiproliferative activities of Rosmarinus officinalis essential oil.

Essential oil was extracted from dried plant by hydrodistillation, and then analyzed by gas chromatography-mass spectrometry (GC/MS). The antibacterial activity of the essential oil was tested by micro-atmosphere technique and agar diffusion against seven strains bacteria. The antiproliferative activity was determined by viability test against P3X63Ag8 myeloma cell in presence of various amount of essential oil. The morphological alterations were searched after staining of treated cells by le May-Grünwald-Giemsa.

Our results showed that the major component detected in R. officinalis essential oil is 1.8-cineole (42%). This essential oil revealed an important antibacterial activity against resistant bacteria. The viability test showed a cytotoxic effect of essential oil on P3X63Ag8 myeloma cell. The morphological of the treated tumor cells analysis revealed a cellular lysis with membrane rupture, a hypertrophy and chromatin condensation.

In conclusion, the R. officinalis essential oil could have an antibacterial activity against resistant bacteria and antiprolioferative activity against myeloma cells.


antibacterial activity, antiproliferative activity, essential oil, Rosmarinus officinalis.

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ISSN: 2458-5920