Antioxidant and bio-activities of leaf extracts from Eucalyptus globulus

ArunDev Sharma, Manisha Chahal, Inderjeet Kaur

Abstract


Eucalyptus globulusmember of Myrtacaea  family, is one of the important medicinal widely used plant.In this study methanolic leaf extract of Eucalyptus globuluswas studied for the estimation of phytochemical constituents , in-vitro  antioxidant and anti-inflammatory activity . Fresh leaves were ground with methanol used for estimations.  Phytochemical constituents were estimated by total phenolic content and total Flavonoid content determination. Spectroscopic analysis by UV-VIS, FT-IR and fluorescents analysis was also conducted.In vitro anti- inflammatory and antimicrobial activities were also monitored.Phytochemical screening revealed the presence of flavonoids , phenols , carbohydates and saponins. Considerable amount of phenolics (50 mg equivalent par g of dry weight)  and flavonoids (23 mg equivalent par g of dry weight) were detected in extract.    Extract exhibited considerable DPPH (1,1-diphenyl-2-picrylhydrazyl), ABTS (2,2’-azinobis-3-ethylbenzothiazoline-6-sulphonate) scavenging activities, Hydroxyl radical scavenging activity, Nitric oxide radical scavenging activity , Iron reducing power  and Superoxide radical scavenging activity.   Fluorescent spectroscopy revealed the presence of fluorophores in extracts. A strong anti-inflammatory activity of extract was observed using fluorescent spectroscopy. An appreciable in vitro antibacterial activity against gram-negative bacteria like gram-negative bacteria Escherichia coli, MTCC 40 was detected.

This study confirmed that methanolic extract Eucalyptusglobuluspossesses potent antioxidant  anti-inflammatory and antibacterial activities possibly important quantity of flavonoid and phenolic content present in it .

 


Keywords


Eucalyptusglobules, flavonoids, reducing power, FT-IR, Fluorescent analysis, In-vitro antibacterial activity, anti-inflammatory activity

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DOI: https://doi.org/10.48347/IMIST.PRSM/ajmap-v7i3.28443

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ISSN: 2458-5920