Phytochemical screening, quantitative analysis and antioxidant activity of Vitex agnus castus L. (Lamiaceae) from South-East of Morocco

Authors

  • HAMDOUCHa A CHEBLIb B Faculty of science, University Ibn Zohr, Agadir, Morocco.
  • IDRISSI HASSANIa L.M. ASDADIa A. Faculty of science, University Ibn Zohr, Agadir, Morocco.

DOI:

https://doi.org/10.48347/IMIST.PRSM/ajmap-v4i1.11369

Keywords:

Phytochemical screening, polyphenols, flavonoids, aglycones, antioxidant activity, Vitex agnus castus

Abstract

This study was designed to assess the phytochemical screening of leaves and seeds of Vitex agnus castus harvested south-East of Morocco and to evaluate their potential antioxydante activity properties using 2, 2 diphenyl-1-picrylehydrazil radical scavenging. Methanol 80% extracts of leaves and seeds were obtained by maceration. Phytochemical screening, quantitative determination of total polyphenols, total flavonoids, Aglycones and anthocyanins contents were assessed. Antioxidant activity was tested using 2, 2 diphenyl-1-picrylehydrazil radical scavenging. Phytochemical screening revealed the presence of alkaloids, terpenes and catechic tannins in leaves and seeds, anthraquinones in seeds and saponins in leaves. Leaves and seeds showed a high content of total polyphenols (574.895 ± 4.600 mg GAE/g dried extract and 551.895 ± 2.300 mg GAE/g dried extract respectively); Total flavonoids content was high in leaves. Quantification of aglycones revealed an important amount of aglycones in leaves (0.118 ± 0.032 mg QE/g dried plant material). Thin layer chromatography revealed a wide variety of flavonoids and the presence of kaempferol in seeds. Results of the present work suggest that there is strong pharmacological potential in developing V. agnus castus as a drug to be used in antioxidant activity

Author Biographies

HAMDOUCHa A CHEBLIb B, Faculty of science, University Ibn Zohr, Agadir, Morocco.

Laboratory of plant biotechnology, 
Laboratory of biology,

IDRISSI HASSANIa L.M. ASDADIa A., Faculty of science, University Ibn Zohr, Agadir, Morocco.

Laboratory of plant biotechnology, 
Laboratory of biology,

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Published

09-02-2018

Issue

Section

Research Articles