ASSESSMENT OF THE ADSORPTION OF AEROMONAS HYDROPHILA ON POLYTHENE IN SODIUM HYPOCHLORITE AND HYDROGEN PEROXIDE TREATED WATER

Chretien LONTSI DJIMELI, A. TAMSA ARFAO, O. NOAH EWOTI, V. ROSSI, E. LANGO-YAYA, V. RASPAL, Moise NOLA, T. SIME-NGANDO

Abstract


A study was carried out on Aeromonas hydrophila adhesion to polythene fragment at different cell growth phases in aquatic microcosm. The main purpose of this study was to assess adsorption capacity, adsorption intensity and kinetics of Aeromonas hydrophila on polythene in sodium hypochlorite (NaOCl) and hydrogen peroxide (H2O2) disinfected water. The mean abundance of adhered A. hydrophila sometimes reached 28 and 111CFU.cm-2 respectively in NaOCl and H2O2 treated water. The adsorption capacities of A. hydrophila cells fluctuated between 1 and 2.12x1057 and between 1 and 2.29x1027 adhered cells.cm-2 in NaOCl and H2O2 disinfected water respectively. The adsorption intensities of this bacterium ranged from -45.81 to 3.49x1019 and from 0.16 to 2.29x1027 respectively in NaOCl and H2O2 treated water. Adsorption capacity and adsorption intensity of A. hydrophila cells on polythene in NaOCl treated water were greater than that obtained in water disinfected with H2O2. Adhesion kinetics of A. hydrophila cells oscillated between 0.001 and 0.930 adhered cell.cm-2.h-1. Adsorption kinetics seem resulted from interactions between bacterial cells and substrates, and depended both on the fragment type and bacterial species. The values of A. hydrophila surface hydrophobicity fluctuated between 65.11 and 92.74% in NaOCl disinfected water and between 63.38 and 89.41% in H2O2 treated water. The mean values of contact angle ranged from 83.1±0.6 to 85.8±0.8°.

   Adhesion of A. hydrophila on polythene is mediated amongst others by cell growth phases, cell surface hydrophobicity, cell kinetic adhesion, adsorption capacity and adsorption intensity.



Keywords


Adsorption capacity, Adsorption intensity, Kinetic adhesion, Disinfectants, Cell growth phases, A. hydrophila.

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ISSN-E: 2509-0445

ISSNPrint: 2605-6070